• Western blot analysis of extracts of various cell lines, using CD248 antibody (STJ114576) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using CD248 antibody (STJ114576) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD248 antibody (18-150) (STJ114576)

SKU:
STJ114576

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD248 (18-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD248
Gene ID: 57124
Uniprot ID: CD248_HUMAN
Immunogen Region: 18-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-150 of human CD248 (NP_065137.1).
Immunogen Sequence: QDPWAAEPRAACGPSSCYAL FPRRRTFLEAWRACRELGGD LATPRTPEEAQRVDSLVGAG PASRLLWIGLQRQARQCQLQ RPLRGFTWTTGDQDTAFTNW AQPASGGPCPAQRCVALEAS GEHRWLEGSCTLA
Tissue Specificity Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.
Post Translational Modifications O-glycosylated with sialylated oligosaccharides. May be N-glycosylated.
Function May play a role in tumor angiogenesis.
Protein Name Endosialin
Tumor Endothelial Marker 1
Cd Antigen Cd248
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Endosialin antibody
Anti-Tumor Endothelial Marker 1 antibody
Anti-Cd Antigen Cd248 antibody
Anti-CD248 antibody
Anti-CD164L1 antibody
Anti-TEM1 antibody

Information sourced from Uniprot.org

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