Anti-CD244 antibody (Extracellular region) [M053] (STJA0005334)

SKU:
STJA0005334-100

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Host: Mouse
Applications: WB/IP/ICC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-CD244/2B4/SLAMF4 M053 (Extracellular region) is suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: M053
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Protein G Purified
Dilution Range: WB 1:500
IHC
ICC 1:200
IP 1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD244
Gene ID: 51744
Uniprot ID: CD244_HUMAN
Immunogen Region: Extracellular region
Specificity: Clone M053 mouse monoclonal antibody detects a 110 kDa* protein on SDS-PAGE Native" or denatured immunoblots of human THP1 monocytes. The antibody prefers the native CD244 protein, but also detects denatured CD244. The antibody works for western blot
Immunogen: Clone (M053) was generated from a recombinant protein that included the extracellular region of human CD244 protein.
Tissue Specificity Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed in all natural killer (NK) cells, monocytes and basophils, TCR-gamma/delta+ T-cells, monocytes, basophils, and on a subset of CD8(+) T-cells.
Post Translational Modifications N-linked glycosylation is essential for the binding to its ligand CD48. Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding. Phosphorylated by FYN and CSK on tyrosine residues following activation. Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells.
Function Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family.its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor. Activating function implicates association with SH2D1A and FYN. Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2. Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis. Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells. Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1. At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells. Involved in the regulation of CD8(+) T-cell proliferation.expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells. Inhibits inflammatory responses in dendritic cells (DCs).
Protein Name Natural Killer Cell Receptor 2b4
Nk Cell Activation-Inducing Ligand
Nail
Nk Cell Type I Receptor Protein 2b4
Nkr2b4
H2b4
Slam Family Member 4
Slamf4
Signaling Lymphocytic Activation Molecule 4
Cd Antigen Cd244
Database Links Reactome: R-HSA-202733
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cell Membrane
Receptor Engagement Results In A Recruitment To Lipid Drafts Essential For The Subsequent Tyrosine Phosphorylation Of The Itsms
Alternative Antibody Names Anti-Natural Killer Cell Receptor 2b4 antibody
Anti-Nk Cell Activation-Inducing Ligand antibody
Anti-Nail antibody
Anti-Nk Cell Type I Receptor Protein 2b4 antibody
Anti-Nkr2b4 antibody
Anti-H2b4 antibody
Anti-Slam Family Member 4 antibody
Anti-Slamf4 antibody
Anti-Signaling Lymphocytic Activation Molecule 4 antibody
Anti-Cd Antigen Cd244 antibody
Anti-CD244 antibody
Anti-2B4 antibody

Information sourced from Uniprot.org

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