• Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD24 Rabbit polyclonal antibody (STJ22979) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CD24 antibody (STJ22979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD24 antibody (1-80) (STJ22979)

SKU:
STJ22979

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD24 (1-80) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD24
Gene ID: 100133941
Uniprot ID: CD24_HUMAN
Immunogen Region: 1-80
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human CD24 (NP_037362.1).
Immunogen Sequence: MGRAMVARLGLGLLLLALLL PTQIYSSETTTGTSSNSSQS TSNSGLAPNPTNATTKAAGG ALQSTASLFVVSLSLLHLYS
Tissue Specificity B-cells. Expressed in a number of B-cell lines including P32/ISH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
Post Translational Modifications Extensively O-glycosylated.
Function May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Plays a role in the control of autoimmunity.
Protein Name Signal Transducer Cd24
Small Cell Lung Carcinoma Cluster 4 Antigen
Cd Antigen Cd24
Database Links Reactome: R-HSA-373760
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Signal Transducer Cd24 antibody
Anti-Small Cell Lung Carcinoma Cluster 4 Antigen antibody
Anti-Cd Antigen Cd24 antibody
Anti-CD24 antibody
Anti-CD24A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance