• Immunofluorescence analysis of Raji cells using CD22 Rabbit polyclonal antibody (STJ28354) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CD22 antibody (STJ28354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of Raji cells, using CD22 antibody (STJ28354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD22 antibody (708-847) (STJ28354)

SKU:
STJ28354

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD22 (708-847) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD22
Gene ID: 933
Uniprot ID: CD22_HUMAN
Immunogen Region: 708-847
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 708-847 of human CD22 (NP_001762.2).
Immunogen Sequence: LQRRWKRTQSQQGLQENSSG QSFFVRNKKVRRAPLSEGPH SLGCYNPMMEDGISYTTLRF PEMNIPRTGDAESSEMQRPP PDCDDTVTYSALHKRQVGDY ENVIPDFPEDEGIHYSELIQ FGVGERPQAQENVDYVILKH
Tissue Specificity B-lymphocytes.
Post Translational Modifications Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2. Phosphorylated on tyrosine residues by LYN.
Function Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins.one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
Protein Name B-Cell Receptor Cd22
B-Lymphocyte Cell Adhesion Molecule
Bl-Cam
Sialic Acid-Binding Ig-Like Lectin 2
Siglec-2
T-Cell Surface Antigen Leu-14
Cd Antigen Cd22
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-5690714
Reactome: R-HSA-983695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-B-Cell Receptor Cd22 antibody
Anti-B-Lymphocyte Cell Adhesion Molecule antibody
Anti-Bl-Cam antibody
Anti-Sialic Acid-Binding Ig-Like Lectin 2 antibody
Anti-Siglec-2 antibody
Anti-T-Cell Surface Antigen Leu-14 antibody
Anti-Cd Antigen Cd22 antibody
Anti-CD22 antibody
Anti-SIGLEC2 antibody

Information sourced from Uniprot.org

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