Anti-CD22 antibody (22-151aa) [SRM13409] (STJ11108139)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-151 of mouse CD22 (NP_033975.3).
General Information
| Short Description | Rabbit monoclonal CD22 (22-151aa) antibody for use in WB, IF-P, IHC-P, FC and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF-P/IHC-P/FC/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM13409 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:3000IF-P, 1:200-1:800IHC-P, 1:500-1:2000FC, 1:100-1:500ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Cd22 |
| Gene ID | 12483 |
| Uniprot ID | CD22_MOUSE |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 22-151 of mouse CD22 (NP_033975.3). |
| Immunogen Region | 22-151aa |
| Immunogen Sequence | Mouse |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine residues by LYN. Phosphorylation of Tyr-783 and Tyr-843 are involved in binding to SYK. Phosphorylation of Tyr-828 is involved in binding to GRB2. Phosphorylation of Tyr-863 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2. |
| Function | Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow. Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration. Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling. If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced. |
| Protein Name | B-Cell Receptor Cd22B-Lymphocyte Cell Adhesion MoleculeBl-CamSialic Acid-Binding Ig-Like Lectin 2Siglec-2T-Cell Surface Antigen Leu-14Cd Antigen Cd22 |
| Database Links | Reactome: R-MMU-198933Reactome: -MMU-5690714Reactome: -MMU-983695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-B-Cell Receptor Cd22 antibodyAnti-B-Lymphocyte Cell Adhesion Molecule antibodyAnti-Bl-Cam antibodyAnti-Sialic Acid-Binding Ig-Like Lectin 2 antibodyAnti-Siglec-2 antibodyAnti-T-Cell Surface Antigen Leu-14 antibodyAnti-Cd Antigen Cd22 antibodyAnti-Cd22 antibodyAnti-Lyb-8 antibodyAnti-Siglec2 antibody |
Information sourced from Uniprot.org