| Host: | Mouse |
| Applications: | FC/IF/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-CD19 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | FR10A7 |
| Conjugation: | Unconjugated |
| Isotype: | IgMk |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Purification: | Affinity purified from tissue culture. |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: | Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30-60 min at RT; information on a suitable antigen retrieval method for staining of formalin-fixed tissues is unavailable to |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: | CD19 |
| Gene ID: | 930 |
| Uniprot ID: | CD19_HUMAN |
| Immunogen: | A BALB/c mouse was immunized with RAJI cells. Fusion partner: X63AG8.653. |
| Post Translational Modifications | Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels. |
| Function | Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge. |
| Protein Name | B-Lymphocyte Antigen Cd19 B-Lymphocyte Surface Antigen B4 Differentiation Antigen Cd19 T-Cell Surface Antigen Leu-12 Cd Antigen Cd19 |
| Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-198933 Reactome: R-HSA-2219530 Reactome: R-HSA-6811558 Reactome: R-HSA-977606 Reactome: R-HSA-983695 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Membrane Raft |
| Alternative Antibody Names | Anti-B-Lymphocyte Antigen Cd19 antibody Anti-B-Lymphocyte Surface Antigen B4 antibody Anti-Differentiation Antigen Cd19 antibody Anti-T-Cell Surface Antigen Leu-12 antibody Anti-Cd Antigen Cd19 antibody Anti-CD19 antibody |
Information sourced from Uniprot.org