| Host: |
Mouse |
| Applications: |
FC/IF/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-CD19 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
EBS-CD-015 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1k |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
Flow Cytometry (0, 5-2, 0 Mu g/million cells in 0, 1ml for 30 min, at 4°C). Immunofluorescence (0, 5-2, 0 Mu g/ml). Immunohistology (1-2 Mu g/ml for 30 min at RT; no suitable antigen retrieval procedure is known to date for formalin-fixed tissues). |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
Cd19 |
| Gene ID: |
12478 |
| Uniprot ID: |
CD19_MOUSE |
| Immunogen: |
A BALB/c mouse was immunized with NALM1 + NALM16 cells. Fusion partner: Sp2/0. |
| Post Translational Modifications | Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-522 is extremely rapid, followed by phosphorylation at Tyr-402. In contrast, phosphorylation of Tyr-493 appears more slowly and is more transient, returning rapidly to basal levels. |
| Function | Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge. |
| Protein Name | B-Lymphocyte Antigen Cd19Differentiation Antigen Cd19Cd Antigen Cd19 |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-198933Reactome: -MMU-6811558Reactome: -MMU-977606Reactome: -MMU-983695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative Antibody Names | Anti-B-Lymphocyte Antigen Cd19 antibodyAnti-Differentiation Antigen Cd19 antibodyAnti-Cd Antigen Cd19 antibodyAnti-Cd19 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
