Anti-CD18 antibody [68-5A5] (STJ16101090)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
ImmunogenA BALB/c mouse was immunized with stimulated human leucocytes. Fusion partner: NS-1.
General Information
| Short Description | Mouse monoclonal anti-CD18 for use in FC, FUNC, WB, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/FUNC/WB/IF/IHC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 68-5A5 |
| Isotype | IgG2ak |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Functional studies (0, 02-2, 0 µg/ml without azide). Immunoblotting (1, 0-2, 0 µg/ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate antigen r |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Gene Symbol | ITGB2 |
| Gene ID | 3689 |
| Uniprot ID | ITB2_HUMAN |
| Immunogen | A BALB/c mouse was immunized with stimulated human leucocytes. Fusion partner: NS-1. |
Additional Info
| Tissue Specificity | Leukocytes. Expressed in neutrophils (at protein level). |
| Post Translational Modifications | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. |
| Function | Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15.the interaction is mediated by ITGAL. Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages. In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. |
| Protein Name | Integrin Beta-2Cell Surface Adhesion Glycoproteins Lfa-1/Cr3/P150 -95 Subunit BetaComplement Receptor C3 Subunit BetaCd Antigen Cd18 |
| Database Links | Reactome: R-HSA-166016Reactome: R-HSA-198933Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-6785807Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative Antibody Names | Anti-Integrin Beta-2 antibodyAnti-Cell Surface Adhesion Glycoproteins Lfa-1/Cr3/P150 -95 Subunit Beta antibodyAnti-Complement Receptor C3 Subunit Beta antibodyAnti-Cd Antigen Cd18 antibodyAnti-ITGB2 antibodyAnti-CD18 antibodyAnti-MFI7 antibody |
Information sourced from Uniprot.org