![Immunofluorescence analysis of PC-12 cells using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98056/381601/STJ119184_1__41435.1713149759.jpg?c=1 "Immunofluorescence analysis of PC-12 cells using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98056/381602/STJ119184_2__23262.1713149760.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry analysis of Cyclin B1 in paraffin-embedded rat ovary tissue using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at a dilution of 1:150 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98056/381603/STJ119184_3__10309.1713149761.jpg?c=1 "Immunohistochemistry analysis of Cyclin B1 in paraffin-embedded rat ovary tissue using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at a dilution of 1:150 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of Cyclin B1 in paraffin-embedded human placenta tissue using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at a dilution of 1:150 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98056/381604/STJ119184_4__35803.1713149762.jpg?c=1 "Immunohistochemistry analysis of Cyclin B1 in paraffin-embedded human placenta tissue using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at a dilution of 1:150 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98056/381605/STJ119184_5__97952.1713149762.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunofluorescence analysis of PC-12 cells using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/98056/381601/STJ119184_1__41435.1713149759.jpg?c=1 "Immunofluorescence analysis of PC-12 cells using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CCNB1 |
| Gene ID: | 891 |
| Uniprot ID: | CCNB1_HUMAN |
| Immunogen Region: | 350-433 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 350-433 of human CCNB1 (NP_114172.1). |
| Immunogen Sequence: | CLALKILDNGEWTPTLQHYL SYTEESLLPVMQHLAKNVVM VNQGLTKHMTVKNKYATSKH AKISTLPQLNSALVQDLAKA VAKV |
| Post Translational Modifications | Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Deubiquitinated by USP22 during G2/M phase. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. |
| Function | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
| Protein Name | G2/Mitotic-Specific Cyclin-B1 |
| Database Links | Reactome: R-HSA-113507 Reactome: R-HSA-156711 Reactome: R-HSA-162658 Reactome: R-HSA-174048 Reactome: R-HSA-176408 Reactome: R-HSA-176412 Reactome: R-HSA-176417 Reactome: R-HSA-2299718 Reactome: R-HSA-2465910 Reactome: R-HSA-2500257 Reactome: R-HSA-2514853 Reactome: R-HSA-2565942 Reactome: R-HSA-2980767 Reactome: R-HSA-2995383 Reactome: R-HSA-3301854 Reactome: R-HSA-4419969 Reactome: R-HSA-6804114 Reactome: R-HSA-68875 Reactome: R-HSA-69273 Reactome: R-HSA-69478 Reactome: R-HSA-75035 Reactome: R-HSA-8852276 Reactome: R-HSA-8878166 Reactome: R-HSA-9725371 Reactome: R-HSA-9825892 |
| Cellular Localisation | Cytoplasm Nucleus Cytoskeleton Microtubule Organizing Center Centrosome |
| Alternative Antibody Names | Anti-G2/Mitotic-Specific Cyclin-B1 antibody Anti-CCNB1 antibody Anti-CCNB antibody |
Information sourced from Uniprot.org