| Host: | Rabbit |
| Applications: | WB/IHC/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-CCNB1 (350-433) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CCNB1 |
| Gene ID: | 891 |
| Uniprot ID: | CCNB1_HUMAN |
| Immunogen Region: | 350-433 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-433 of human CCNB1 (NP_114172.1). |
| Immunogen Sequence: | CLALKILDNGEWTPTLQHYL SYTEESLLPVMQHLAKNVVM VNQGLTKHMTVKNKYATSKH AKISTLPQLNSALVQDLAKA VAKV |
| Post Translational Modifications | Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. |
| Function | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
| Protein Name | G2/Mitotic-Specific Cyclin-B1 |
| Database Links | Reactome: R-HSA-113507Reactome: R-HSA-156711Reactome: R-HSA-162658Reactome: R-HSA-174048Reactome: R-HSA-176408Reactome: R-HSA-176412Reactome: R-HSA-176417Reactome: R-HSA-2299718Reactome: R-HSA-2465910Reactome: R-HSA-2500257Reactome: R-HSA-2514853Reactome: R-HSA-2565942Reactome: R-HSA-2980767Reactome: R-HSA-2995383Reactome: R-HSA-3301854Reactome: R-HSA-4419969Reactome: R-HSA-6804114Reactome: R-HSA-68875Reactome: R-HSA-69273Reactome: R-HSA-69478Reactome: R-HSA-75035Reactome: R-HSA-8852276Reactome: R-HSA-8878166 |
| Cellular Localisation | CytoplasmNucleusCytoskeletonMicrotubule Organizing CenterCentrosome |
| Alternative Antibody Names | Anti-G2/Mitotic-Specific Cyclin-B1 antibodyAnti-CCNB1 antibodyAnti-CCNB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
