• Western blot analysis of lysates from HepG2 cells, using Cyclin B1 Rabbit polyclonal antibody (STJ11103802). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CCNB1 antibody (350-433) (STJ11103802)

SKU:
STJ11103802

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-G2/Mitotic-Specific Cyclin-B1 (350-433) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNB1
Gene ID: 891
Uniprot ID: CCNB1_HUMAN
Immunogen Region: 350-433
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-433 of human Cyclin B1 (NP_114172.1).
Immunogen Sequence: CLALKILDNGEWTPTLQHYL SYTEESLLPVMQHLAKNVVM VNQGLTKHMTVKNKYATSKH AKISTLPQLNSALVQDLAKA VAKV
Post Translational Modifications Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Protein Name G2/Mitotic-Specific Cyclin-B1
Database Links Reactome: R-HSA-113507
Reactome: R-HSA-156711
Reactome: R-HSA-162658
Reactome: R-HSA-174048
Reactome: R-HSA-176408
Reactome: R-HSA-176412
Reactome: R-HSA-176417
Reactome: R-HSA-2299718
Reactome: R-HSA-2465910
Reactome: R-HSA-2500257
Reactome: R-HSA-2514853
Reactome: R-HSA-2565942
Reactome: R-HSA-2980767
Reactome: R-HSA-2995383
Reactome: R-HSA-3301854
Reactome: R-HSA-4419969
Reactome: R-HSA-6804114
Reactome: R-HSA-68875
Reactome: R-HSA-69273
Reactome: R-HSA-69478
Reactome: R-HSA-75035
Reactome: R-HSA-8852276
Reactome: R-HSA-8878166
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Microtubule Organizing Center
Centrosome
Alternative Antibody Names Anti-G2/Mitotic-Specific Cyclin-B1 antibody
Anti-CCNB1 antibody
Anti-CCNB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance