Anti-CCKBR antibody (240-335) (STJ22926)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CCKBR (240-335) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CCKBR |
| Gene ID | 887 |
| Uniprot ID | GASR_HUMAN |
| Immunogen Region | 240-335 |
| Immunogen Sequence | LISRELYLGLRFDGDSDSDS QSRVRNQGGLPGAVHQNGRC RPETGAVGEDSDGCYVQLPR SRPALELTALTAPGPGSGSR PTQAKLLAKKRVVRML |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 240-335 of human CCKBR (NP_795344.1). |
Additional Info
| Tissue Specificity | Isoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC-28 cell lines, not expressed in GLC-19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo. |
| Function | Receptor for gastrin and cholecystokinin. The CCK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism. |
| Protein Name | Gastrin/Cholecystokinin Type B ReceptorCck-B ReceptorCck-BrCholecystokinin-2 ReceptorCck2-R |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-416476Reactome: R-HSA-881907 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Gastrin/Cholecystokinin Type B Receptor antibodyAnti-Cck-B Receptor antibodyAnti-Cck-Br antibodyAnti-Cholecystokinin-2 Receptor antibodyAnti-Cck2-R antibodyAnti-CCKBR antibodyAnti-CCKRB antibody |
Information sourced from Uniprot.org