| Host: |
Mouse |
| Applications: |
WB/ICC/IHC/IP |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Girdin (C-Term) is suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M012 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:250IHC 1:50ICC 1:50IP 1:50 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CCDC88A |
| Gene ID: |
55704 |
| Uniprot ID: |
GRDN_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
Clone M012 mouse monoclonal antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells and human brain tissue. The antibody also works for immmunoprecipitation, immunohistochemistry, and immunocytochemistry. |
| Immunogen: |
Clone (M012) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human Girdin. |
| Tissue Specificity | Expressed ubiquitously. |
| Post Translational Modifications | Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for delocalization from the cell membrane and for cell migration. Phosphorylated on tyrosine residues which promotes binding to phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1/p85a and enhances PI3K activity. Tyrosine-phosphorylated by both receptor and non-receptor tyrosine kinases in vitro. Tyrosine phosphorylation is required for AKT1-dependent phosphorylation of Ser-1417. Phosphorylation at Ser-1690 by PRKCQ disrupts interaction with GNAI3 and inhibits guanine nucleotide exchange factor activity. |
| Function | Bifunctional modulator of guanine nucleotide-binding proteins (G proteins). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G(i) alpha subunits. Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G(s) subunit alpha GNAS. Essential for cell migration. Interacts in complex with G(i) alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration. Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex which enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB. Phosphorylation of AKT1/PKB induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation. Binds in its tyrosine-phosphorylated form to the phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1 which enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration. Plays a role as a key modulator of the AKT-mTOR signaling pathway, controlling the tempo of the process of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Inhibition of G(s) subunit alpha GNAS leads to reduced cellular levels of cAMP and suppression of cell proliferation. Essential for the integrity of the actin cytoskeleton. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. Plays a role in ciliogenesis and cilium morphology and positioning and this may partly be through regulation of the localization of scaffolding protein CROCC/Rootletin. |
| Protein Name | GirdinAkt Phosphorylation EnhancerApeCoiled-Coil Domain-Containing Protein 88aG Alpha-Interacting Vesicle-Associated ProteinGivGirders Of Actin FilamentHook-Related Protein 1Hkrp1 |
| Database Links | Reactome: R-HSA-9696264Reactome: R-HSA-9696273 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmCytosolCytoplasmic VesicleCell ProjectionLamellipodiumCytoskeletonCilium Basal BodyMicrotubule Organizing CenterCentrosomeCentrioleLocalizes To The Cytosol In Unstimulated Cells While Egf Stimulation Promotes Membrane Localization And Guanine Nucleotide Exchange Factor ActivityLocalizes To The Cell Membrane Through Interaction With Phosphoinositides |
| Alternative Antibody Names | Anti-Girdin antibodyAnti-Akt Phosphorylation Enhancer antibodyAnti-Ape antibodyAnti-Coiled-Coil Domain-Containing Protein 88a antibodyAnti-G Alpha-Interacting Vesicle-Associated Protein antibodyAnti-Giv antibodyAnti-Girders Of Actin Filament antibodyAnti-Hook-Related Protein 1 antibodyAnti-Hkrp1 antibodyAnti-CCDC88A antibodyAnti-APE antibodyAnti-GRDN antibodyAnti-KIAA1212 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
