| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CBFB (1-182) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CBFB |
| Gene ID: |
865 |
| Uniprot ID: |
PEBB_HUMAN |
| Immunogen Region: |
1-182 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human CBFB (NP_001746.1). |
| Immunogen Sequence: |
MPRVVPDQRSKFENEEFFRK LSRECEIKYTGFRDRPHEER QARFQNACRDGRSEIAFVAT GTNLSLQFFPASWQGEQRQT PSREYVDLEREAGKVYLKAP MILNGVCVIWKGWIDLQRLD GMGCLEFDEERAQQEDALAQ QAFEEARRRTREFEDRDRSH REEMEVRVSQLLAVTGKKTT RP |
| Function | Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. |
| Protein Name | Core-Binding Factor Subunit BetaCbf-BetaPolyomavirus Enhancer-Binding Protein 2 Beta SubunitPea2-BetaPebp2-BetaSl3-3 Enhancer Factor 1 Subunit BetaSl3/Akv Core-Binding Factor Beta Subunit |
| Database Links | Reactome: R-HSA-8877330Reactome: R-HSA-8878166Reactome: R-HSA-8931987Reactome: R-HSA-8934593Reactome: R-HSA-8935964Reactome: R-HSA-8936459Reactome: R-HSA-8939236Reactome: R-HSA-8939242Reactome: R-HSA-8939243Reactome: R-HSA-8939245Reactome: R-HSA-8939246Reactome: R-HSA-8939247Reactome: R-HSA-8939256Reactome: R-HSA-8939902Reactome: R-HSA-8940973Reactome: R-HSA-8941284Reactome: R-HSA-8941326Reactome: R-HSA-8941332Reactome: R-HSA-8941333Reactome: R-HSA-8941858Reactome: R-HSA-8949275Reactome: R-HSA-8951911Reactome: R-HSA-8951936Reactome: R-HSA-9018519Reactome: R-HSA-9616222 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Core-Binding Factor Subunit Beta antibodyAnti-Cbf-Beta antibodyAnti-Polyomavirus Enhancer-Binding Protein 2 Beta Subunit antibodyAnti-Pea2-Beta antibodyAnti-Pebp2-Beta antibodyAnti-Sl3-3 Enhancer Factor 1 Subunit Beta antibodyAnti-Sl3/Akv Core-Binding Factor Beta Subunit antibodyAnti-CBFB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
