Anti-CASTOR1 antibody (1-329) (STJ11103420)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CASTOR1 (1-329) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CASTOR1 |
| Gene ID | 652968 |
| Uniprot ID | CAST1_HUMAN |
| Immunogen Region | 1-329 |
| Immunogen Sequence | MELHILEHRVRVLSVARPGL WLYTHPLIKLLFLPRRSRCK FFSLTETPEDYTLMVDEEGF KELPPSEFLQVAEATWLVLN VSSHSGAAVQAAGVTKIARS VIAPLAEHHVSVLMLSTYQT DFILVREQDLSVVIHTLAQE FDIYREVGGEPVPVTRDDSS NGFPRTQHGPSPTVHPIQSP QNRFCVLTLDPETLPAIATT LIDVLFYSHSTPKEAASSSP EPSSITFFAFSLIEGYISI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human CASTOR1 (NP_001032755.1). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylation at Ser-14 by AKT1, promoting the interaction between CASTOR1 and RNF167. Ubiquitinated by RNF167 via 'Lys-29'-polyubiquitination, leading to its degradation, releasing the GATOR2 complex. Ubiquitination by RNF167 is promoted by phosphorylation at Ser-14 by AKT1. |
| Function | Functions as an intracellular arginine sensor within the amino acid-sensing branch of the TORC1 signaling pathway. As a homodimer or a heterodimer with CASTOR2, binds and inhibits the GATOR subcomplex GATOR2 and thereby mTORC1. Binding of arginine to CASTOR1 allosterically disrupts the interaction of CASTOR1-containing dimers with GATOR2 which can in turn activate mTORC1 and the TORC1 signaling pathway. |
| Protein Name | Cytosolic Arginine Sensor For Mtorc1 Subunit 1Cellular Arginine Sensor For Mtorc1 Protein 1Gats-Like Protein 3 |
| Database Links | Reactome: R-HSA-9639288 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Cytosolic Arginine Sensor For Mtorc1 Subunit 1 antibodyAnti-Cellular Arginine Sensor For Mtorc1 Protein 1 antibodyAnti-Gats-Like Protein 3 antibodyAnti-CASTOR1 antibodyAnti-GATSL3 antibody |
Information sourced from Uniprot.org