| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Caspase-3 (6-105) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CASP3 |
| Gene ID: |
836 |
| Uniprot ID: |
CASP3_HUMAN |
| Immunogen Region: |
6-105 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 6-105 of human Caspase-3 (NP_004337.2). |
| Immunogen Sequence: |
NSVDSKSIKNLEPKIIHGSE SMDSGISLDNSYKMDYPEMG LCIIINNKNFHKSTGMTSRS GTDVDAANLRETFRNLKYEV RNKNDLTREEIVELMRDVSK |
| Tissue Specificity | Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. |
| Post Translational Modifications | Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. (Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP3 processing, preventing CASP3 activation and ability to recognize and cleave substrates. |
| Function | Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. |
| Protein Name | Caspase-3Casp-3ApopainCysteine Protease Cpp32Cpp-32Protein YamaSrebp Cleavage Activity 1Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 |
| Database Links | Reactome: R-HSA-111459Reactome: R-HSA-111463Reactome: R-HSA-111464Reactome: R-HSA-111465Reactome: R-HSA-111469Reactome: R-HSA-140342Reactome: R-HSA-1474228Reactome: R-HSA-2028269Reactome: R-HSA-205025Reactome: R-HSA-211736Reactome: R-HSA-264870Reactome: R-HSA-351906Reactome: R-HSA-418889Reactome: R-HSA-449836Reactome: R-HSA-5620971 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Caspase-3 antibodyAnti-Casp-3 antibodyAnti-Apopain antibodyAnti-Cysteine Protease Cpp32 antibodyAnti-Cpp-32 antibodyAnti-Protein Yama antibodyAnti-Srebp Cleavage Activity 1 antibodyAnti-Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 antibodyAnti-CASP3 antibodyAnti-CPP32 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of RAW264. 7 cells using [KO Validated] Caspase-3 Rabbit polyclonal antibody (STJ11101178) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/90100/358863/STJ11101178_1__46120.1713123228.jpg?c=1 "Immunofluorescence analysis of RAW264. 7 cells using [KO Validated] Caspase-3 Rabbit polyclonal antibody (STJ11101178) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")