| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CANX |
| Gene ID: | 821 |
| Uniprot ID: | CALX_HUMAN |
| Immunogen Region: | 500-592 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 500-592 of human Calnexin (NP_001737.1). |
| Immunogen Sequence: | LFCCSGKKQTSGMEYKKTDA PQPDVKEEEEEKEEEKDKGD EEEEGEEKLEEKQKSDAEED GGTVSQEEEDRKPKAEEDEI LNRSPRNRKPRRE |
| Post Translational Modifications | Phosphorylated at Ser-564 by MAPK3/ERK1. Phosphorylation by MAPK3/ERK1 increases its association with ribosomes. Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins. Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4. |
| Function | Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse. |
| Protein Name | Calnexin Ip90 Major Histocompatibility Complex Class I Antigen-Binding Protein P88 P90 |
| Database Links | Reactome: R-HSA-168316 Reactome: R-HSA-2132295 Reactome: R-HSA-8984722 Reactome: R-HSA-901042 Reactome: R-HSA-9020956 Reactome: R-HSA-9683686 Reactome: R-HSA-9694548 Reactome: R-HSA-983170 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Type I Membrane Protein Mitochondrion Membrane Melanosome Membrane Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv The Palmitoylated Form Preferentially Localizes To The Perinuclear Rough Er Localizes To Endoplasmic Reticulum Mitochondria-Associated Membrane (Mams) That Connect The Endoplasmic Reticulum And The Mitochondria |
| Alternative Antibody Names | Anti-Calnexin antibody Anti-Ip90 antibody Anti-Major Histocompatibility Complex Class I Antigen-Binding Protein P88 antibody Anti-P90 antibody Anti-CANX antibody |
Information sourced from Uniprot.org