• Immunohistochemistry analysis of paraffin-embedded mouse kidney using Calbindin antibody (STJ22861) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Calbindin antibody (STJ22861) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Calbindin antibody (STJ22861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CALB1 antibody (1-261) (STJ22861)

SKU:
STJ22861

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CALB1 (1-261) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CALB1
Gene ID: 793
Uniprot ID: CALB1_HUMAN
Immunogen Region: 1-261
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human Calbindin (NP_004920.1).
Immunogen Sequence: MAESHLQSSLITASQFFEIW LHFDADGSGYLEGKELQNLI QELQQARKKAGLELSPEMKT FVDQYGQRDDGKIGIVELAH VLPTEENFLLLFRCQQLKSC EEFMKTWRKYDTDHSGFIET EELKNFLKDLLEKANKTVDD TKLAEYTDLMLKLFDSNNDG KLELTEMARLLPVQENFLLK FQGIKMCGKEFNKAFELYDQ DGNGYIDENELDALLKDLCE KNKQDLDINNITTYKKNIM
Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Protein Name Calbindin
Calbindin D28
D-28k
Vitamin D-Dependent Calcium-Binding Protein - Avian-Type
Database Links Reactome: R-HSA-977225
Alternative Antibody Names Anti-Calbindin antibody
Anti-Calbindin D28 antibody
Anti-D-28k antibody
Anti-Vitamin D-Dependent Calcium-Binding Protein - Avian-Type antibody
Anti-CALB1 antibody
Anti-CAB27 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance