| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CACYBP (1-228) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CACYBP |
| Gene ID: |
27101 |
| Uniprot ID: |
CYBP_HUMAN |
| Immunogen Region: |
1-228 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human CACYBP (NP_055227.1). |
| Immunogen Sequence: |
MASEELQKDLEEVKVLLEKA TRKRVRDALTAEKSKIETEI KNKMQQKSQKKAELLDNEKP AAVVAPITTGYTVKISNYGW DQSDKFVKIYITLTGVHQVP TENVQVHFTERSFDLLVKNL NGKSYSMIVNNLLKPISVEG SSKKVKTDTVLILCRKKVEN TRWDYLTQVEKECKEKEKPS YDTETDPSEGLMNVLKKIYE DGDDDMKRTINKAWVESREK QAKGDTEF |
| Post Translational Modifications | Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations. |
| Function | May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). |
| Protein Name | Calcyclin-Binding ProteinCacybpHcacybpS100a6-Binding ProteinSiah-Interacting Protein |
| Cellular Localisation | NucleusCytoplasmCytoplasmic At Low Calcium ConcentrationsIn Neuroblastoma CellsAfter A Retinoic Acid (Ra) Induction And Calcium IncreaseIt Localizes In Both The Nucleus And CytoplasmThe Nuclear Fraction May Be Phosphorylated |
| Alternative Antibody Names | Anti-Calcyclin-Binding Protein antibodyAnti-Cacybp antibodyAnti-Hcacybp antibodyAnti-S100a6-Binding Protein antibodyAnti-Siah-Interacting Protein antibodyAnti-CACYBP antibodyAnti-S100A6BP antibodyAnti-SIP antibodyAnti-PNAS-107 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89814/358277/STJ11100880_1__41081.1713122624.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
