Western blot analysis of extracts of various cell lines, using CACNA2D3 antibody (STJ112353) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CACNA2D3 antibody (900-1040) (STJ112353)

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STJ112353
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Short Description :Rabbit polyclonal CACNA2D3 (900-1040) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:1000-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:CACNA2D3
Gene ID:55799
Uniprot ID:CA2D3_HUMAN
Immunogen Region:900-1040
Immunogen Sequence:LYDYQAMCRANKESSDGAHG LLDPYNAFLSAVKWIMTELV LFLVEFNLCSWWHSDMTAKA QKLKQTLEPCDTEYPAFVSE RTIKETTGNIACEDCSKSFV IQQIPSSNLFMVVVDSSCLC ESVAPITMAPIEIRYNESLK C
Specificity:Recombinant fusion protein containing a sequence corresponding to amino acids 900-1040 of human CACNA2D3 (NP_060868.2).
Tissue Specificity Only detected in brain. Not present in lung, testis, aorta, spleen, jejunum, ventricular muscle and kidney (at protein level). it is brain-specific, while it is widely expressed.
Post Translational Modifications N-glycosylated. May be proteolytically processed into subunits alpha-2-3 and delta-3 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role.
Function The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) but not T-type (CACNA1G).
Protein Name Voltage-Dependent Calcium Channel Subunit Alpha-2/Delta-3
Voltage-Gated Calcium Channel Subunit Alpha-2/Delta-3 Cleaved Into - Voltage-Dependent Calcium Channel Subunit Alpha-2-3 - Voltage-Dependent Calcium Channel Subunit Delta-3
Database Links Reactome: R-HSA-112308
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Voltage-Dependent Calcium Channel Subunit Alpha-2/Delta-3 antibody
Anti-Voltage-Gated Calcium Channel Subunit Alpha-2/Delta-3 Cleaved Into - Voltage-Dependent Calcium Channel Subunit Alpha-2-3 - Voltage-Dependent Calcium Channel Subunit Delta-3 antibody
Anti-CACNA2D3 antibody

Information sourced from Uniprot.org

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