Western blot analysis of extracts of various cell lines, using CACNA2D3 antibody (STJ112353) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CACNA2D3 antibody (900-1040) (STJ112353)

SKU:
STJ112353
€140.63 - €655.63
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CACNA2D3
Gene ID: 55799
Uniprot ID: CA2D3_HUMAN
Immunogen Region: 900-1040
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 900-1040 of human CACNA2D3 (NP_060868.2).
Immunogen Sequence: LYDYQAMCRANKESSDGAHG LLDPYNAFLSAVKWIMTELV LFLVEFNLCSWWHSDMTAKA QKLKQTLEPCDTEYPAFVSE RTIKETTGNIACEDCSKSFV IQQIPSSNLFMVVVDSSCLC ESVAPITMAPIEIRYNESLK C
Tissue Specificity Only detected in brain. Not present in lung, testis, aorta, spleen, jejunum, ventricular muscle and kidney (at protein level). it is brain-specific, while it is widely expressed.
Post Translational Modifications N-glycosylated. May be proteolytically processed into subunits alpha-2-3 and delta-3 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role.
Function The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) but not T-type (CACNA1G).
Protein Name Voltage-Dependent Calcium Channel Subunit Alpha-2/Delta-3
Voltage-Gated Calcium Channel Subunit Alpha-2/Delta-3 Cleaved Into - Voltage-Dependent Calcium Channel Subunit Alpha-2-3 - Voltage-Dependent Calcium Channel Subunit Delta-3
Database Links Reactome: R-HSA-112308
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Voltage-Dependent Calcium Channel Subunit Alpha-2/Delta-3 antibody
Anti-Voltage-Gated Calcium Channel Subunit Alpha-2/Delta-3 Cleaved Into - Voltage-Dependent Calcium Channel Subunit Alpha-2-3 - Voltage-Dependent Calcium Channel Subunit Delta-3 antibody
Anti-CACNA2D3 antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJ11106578 Anti-ATN1 antibody (900-1040 aa) (STJ11106578) Rabbit Polyclonal Human WB/IF/ICC/ELISA
STJ13100087 Anti-CACNA2D3 antibody (STJ13100087) Rabbit Polyclonal Mouse/Rat IHC/WB
STJ13100086 Anti-CACNA2D3 antibody (STJ13100086) Rabbit Polyclonal Mouse/Rat IHC/WB
STJ13100899 Anti-CACNA2D3 antibody (Internal Region) (STJ13100899) Goat Polyclonal Human/Mouse/Rat IHC/WB
STJ197410 Anti-CACNA2D3 antibody (500-580 aa) (STJ197410) Rabbit Polyclonal Human/Rat/Mouse WB
!