Anti-CACNA1S antibody (N-Term) (STJ13100865)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from n-terminal of human CACNA1S (Cav1.1) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
General Information
| Short Description | Rabbit polyclonal anti-CACNA1S (N-Term) for use in IHC and WB in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:1000 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | CACNA1S |
| Gene ID | 779 |
| Uniprot ID | CAC1S_HUMAN |
| Immunogen | A synthetic peptide from n-terminal of human CACNA1S (Cav1.1) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Immunogen Region | N-Term |
| Specificity | Specific for CACNA1S. |
Additional Info
| Post Translational Modifications | The alpha-1S subunit is found in two isoforms in the skeletal muscle: a minor form of 212 kDa containing the complete amino acid sequence, and a major form of 190 kDa derived from the full-length form by post-translational proteolysis close to Phe-1690. Phosphorylated. Phosphorylation by PKA activates the calcium channel. Both the minor and major forms are phosphorylated in vitro by PKA. Phosphorylation at Ser-1575 is involved in beta-adrenergic-mediated regulation of the channel. |
| Function | Pore-forming, alpha-1S subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents in skeletal muscle. Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle via their interaction with RYR1, which triggers Ca(2+) release from the sarcoplasmic reticulum and ultimately results in muscle contraction. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. |
| Protein Name | Voltage-Dependent L-Type Calcium Channel Subunit Alpha-1sCalcium Channel - L Type - Alpha-1 Polypeptide - Isoform 3 - Skeletal MuscleVoltage-Gated Calcium Channel Subunit Alpha Cav1.1 |
| Database Links | Reactome: R-HSA-419037 |
| Cellular Localisation | Cell MembraneSarcolemmaT-TubuleMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Voltage-Dependent L-Type Calcium Channel Subunit Alpha-1s antibodyAnti-Calcium Channel - L Type - Alpha-1 Polypeptide - Isoform 3 - Skeletal Muscle antibodyAnti-Voltage-Gated Calcium Channel Subunit Alpha Cav1.1 antibodyAnti-CACNA1S antibodyAnti-CACH1 antibodyAnti-CACN1 antibodyAnti-CACNL1A3 antibody |
Information sourced from Uniprot.org