• Immunohistochemistry analysis of paraffin-embedded rat heart using CA3 Antibody (STJ22857) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using CA3 Antibody (STJ22857) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CA3 antibody (STJ22857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of Rat skeletal muscle, using CA3 antibody (STJ22857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CA3 antibody (1-260) (STJ22857)

SKU:
STJ22857

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CA3 (1-260) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CA3
Gene ID: 761
Uniprot ID: CAH3_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CA3 (NP_005172.1).
Immunogen Sequence: MAKEWGYASHNGPDHWHELF PNAKGENQSPVELHTKDIRH DPSLQPWSVSYDGGSAKTIL NNGKTCRVVFDDTYDRSMLR GGPLPGPYRLRQFHLHWGSS DDHGSEHTVDGVKYAAELHL VHWNPKYNTFKEALKQRDGI AVIGIFLKIGHENGEFQIFL DALDKIKTKGKEAPFTKFDP SCLFPACRDYWTYQGSFTTP PCEECIVWLLLKEPMTVSSD QMAKLRSLLSSAENEPPVP
Tissue Specificity Muscle specific.
Post Translational Modifications S-thiolated both by thiol-disulfide exchange with glutathione disulfide and by oxyradical-initiated S-thiolation with reduced glutathione. S-glutathionylated in hepatocytes under oxidative stress.
Function Reversible hydration of carbon dioxide.
Protein Name Carbonic Anhydrase 3
Carbonate Dehydratase Iii
Carbonic Anhydrase Iii
Ca-Iii
Database Links Reactome: R-HSA-1475029
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Carbonic Anhydrase 3 antibody
Anti-Carbonate Dehydratase Iii antibody
Anti-Carbonic Anhydrase Iii antibody
Anti-Ca-Iii antibody
Anti-CA3 antibody

Information sourced from Uniprot.org

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