| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CA3 |
| Gene ID: | 761 |
| Uniprot ID: | CAH3_HUMAN |
| Immunogen Region: | 1-260 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CA3 (NP_005172.1). |
| Immunogen Sequence: | MAKEWGYASHNGPDHWHELF PNAKGENQSPVELHTKDIRH DPSLQPWSVSYDGGSAKTIL NNGKTCRVVFDDTYDRSMLR GGPLPGPYRLRQFHLHWGSS DDHGSEHTVDGVKYAAELHL VHWNPKYNTFKEALKQRDGI AVIGIFLKIGHENGEFQIFL DALDKIKTKGKEAPFTKFDP SCLFPACRDYWTYQGSFTTP PCEECIVWLLLKEPMTVSSD QMAKLRSLLSSAENEPPVP |
| Tissue Specificity | Muscle specific. |
| Post Translational Modifications | S-thiolated both by thiol-disulfide exchange with glutathione disulfide and by oxyradical-initiated S-thiolation with reduced glutathione. S-glutathionylated in hepatocytes under oxidative stress. |
| Function | Reversible hydration of carbon dioxide. |
| Protein Name | Carbonic Anhydrase 3Carbonate Dehydratase IiiCarbonic Anhydrase IiiCa-Iii |
| Database Links | Reactome: R-HSA-1475029 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Carbonic Anhydrase 3 antibodyAnti-Carbonate Dehydratase Iii antibodyAnti-Carbonic Anhydrase Iii antibodyAnti-Ca-Iii antibodyAnti-CA3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
