Anti-C8B antibody (371-420 aa) (STJ98631)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human C8B at the amino acid range 371-420
General Information
| Short Description | Rabbit polyclonal anti-Complement component C8 beta chain (371-420 aa) for use in WB and ELISA in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:10000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | C8B |
| Gene ID | 732 |
| Uniprot ID | CO8B_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human C8B at the amino acid range 371-420 |
| Immunogen Region | 371-420 aa |
| Specificity | C8 Beta Polyclonal Antibody detects endogenous levels of C8 Beta |
Additional Info
| Function | Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C8B, together with C8A and C8G, inserts into the target membrane, but does not form pores by itself. During MAC assembly, associates with C5b, C6 and C7 to form the C5b8 intermediate complex that inserts into the target membrane and traverses the bilayer increasing membrane rigidity. |
| Protein Name | Complement Component C8 Beta ChainComplement Component 8 Subunit Beta |
| Database Links | Reactome: R-HSA-166665Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedTarget Cell MembraneMulti-Pass Membrane ProteinSecreted As Soluble ProteinInserts Into The Cell Membrane Of Target Cells |
| Alternative Antibody Names | Anti-Complement Component C8 Beta Chain antibodyAnti-Complement Component 8 Subunit Beta antibodyAnti-C8B antibody |
Information sourced from Uniprot.org