• STJ72223 (0. 01µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
  • STJ72223 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ72223 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • STJ72223 (6µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
  • STJ72223 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Anti-C5AR1/CD88 antibody (C-Term) (STJ72223)

SKU:
STJ72223-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-C5AR1/CD88 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.01-0.03µg/ml
IF-Strong expression of the protein seen in the cytoplasm of HepG2 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: C5AR1
Gene ID: 728
Uniprot ID: C5AR1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_001727.1
Immunogen Sequence: RESKSFTRSTVDT
Function Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.
Protein Name C5a Anaphylatoxin Chemotactic Receptor 1
C5a Anaphylatoxin Chemotactic Receptor
C5a-R
C5ar
Cd Antigen Cd88
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-6798695
Reactome: R-HSA-977606
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle
Phosphorylated C5ar Colocalizes With Arrb1 And Arrb2 In Cytoplasmic Vesicles
Alternative Antibody Names Anti-C5a Anaphylatoxin Chemotactic Receptor 1 antibody
Anti-C5a Anaphylatoxin Chemotactic Receptor antibody
Anti-C5a-R antibody
Anti-C5ar antibody
Anti-Cd Antigen Cd88 antibody
Anti-C5AR1 antibody
Anti-C5AR antibody
Anti-C5R1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance