• STJ71416 (0. 3µg/ml) staining of human lymph node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • HEK293 overexpressing GCNT3 (RC202007) and probed with STJ71416 (mock transfection in first lane) , tested by Origene.

Anti-C2GnT-M antibody (273-284) (STJ71416)

SKU:
STJ71416-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-C2GnT-M (273-284) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: ELISA-antibody detection limit dilution 1:2000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: GCNT3
Gene ID: 9245
Uniprot ID: GCNT3_HUMAN
Immunogen Region: 273-284
Accession Number: NP_004742.1
Immunogen Sequence: RDTLHLTNKKKD
Post Translational Modifications N-glycosylated.
Function Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
Protein Name Beta-1 -3-Galactosyl-O-Glycosyl-Glycoprotein Beta-1 -6-N-Acetylglucosaminyltransferase 3
C2gnt-Mucin Type
C2gnt-M
Hc2gnt-M
Core 2/Core 4 Beta-1 -6-N-Acetylglucosaminyltransferase
C2/4gnt
Database Links Reactome: R-HSA-913709
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Beta-1 -3-Galactosyl-O-Glycosyl-Glycoprotein Beta-1 -6-N-Acetylglucosaminyltransferase 3 antibody
Anti-C2gnt-Mucin Type antibody
Anti-C2gnt-M antibody
Anti-Hc2gnt-M antibody
Anti-Core 2/Core 4 Beta-1 -6-N-Acetylglucosaminyltransferase antibody
Anti-C2/4gnt antibody
Anti-GCNT3 antibody

Information sourced from Uniprot.org

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