Anti-C14orf169/NO66 antibody (Internal) (STJ71098)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-C14orf169/NO66 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 70kDa band observed in nuclear lysates of cell lines A431, HepG2 and NIH3T3 (calculated MW of 71.1kDa according to Human NP_078920.2 and 67.6kDa according to Mouse NP_076122.2) |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | RIOX1 |
| Gene ID | 79697 |
| Uniprot ID | RIOX1_HUMAN |
| Accession Number | NP_078920.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | GAQHSDSKDPR |
Additional Info
| Function | Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. Also catalyzes demethylation of non-histone proteins, such as CGAS: demethylation of monomethylated CGAS promotes interaction between CGAS and PARP1, followed by PARP1 inactivation. Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216', thereby playing a role in ribosome biogenesis. Participates in MYC-induced transcriptional activation. |
| Protein Name | Ribosomal Oxygenase 160s Ribosomal Protein L8 Histidine HydroxylaseBifunctional Lysine-Specific Demethylase And Histidyl-Hydroxylase No66Myc-Associated Protein With Jmjc DomainNucleolar Protein 66Hsno66Ribosomal Oxygenase No66Rox |
| Database Links | Reactome: R-HSA-9629569 |
| Cellular Localisation | NucleusNucleolusNucleoplasmGranular Part Of NucleoliNucleoplasmic FociSome Of Them Associated With Nucleoli |
| Alternative Antibody Names | Anti-Ribosomal Oxygenase 1 antibodyAnti-60s Ribosomal Protein L8 Histidine Hydroxylase antibodyAnti-Bifunctional Lysine-Specific Demethylase And Histidyl-Hydroxylase No66 antibodyAnti-Myc-Associated Protein With Jmjc Domain antibodyAnti-Nucleolar Protein 66 antibodyAnti-Hsno66 antibodyAnti-Ribosomal Oxygenase No66 antibodyAnti-Rox antibodyAnti-RIOX1 antibodyAnti-C14orf169 antibodyAnti-MAPJD antibodyAnti-NO66 antibody |
Information sourced from Uniprot.org