| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BSND |
| Gene ID: |
7809 |
| Uniprot ID: |
BSND_HUMAN |
| Immunogen Region: |
54-320 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 54-320 of human BSND (NP_476517.1). |
| Immunogen Sequence: |
CQCYPKITFVPADSDFQGIL SPKAMGLLENGLAAEMKSPS PQPPYVRLWEEAAYDQSLPD FSHIQMKVMSYSEDHRSLLA PEMGQPKLGTSDGGEGGPGD VQAWMEAAVVIHKGSDESEG ERRLTQSWPGPLACPQGPAP LASFQDDLDMDSSEGSSPNA SPHDREEACSPQQEPQGCRC PLDRFQDFALIDAPTLEDEP QEGQQWEIALPNNWQRYPRT KVEEKEASDTGGEEPEKEE |
| Tissue Specificity | Expressed primarily in kidney. Expressed in specific nephron segments and in the stria vascularis of the inner ear. |
| Post Translational Modifications | Palmitoylation is necessary for activation of plasma membrane-inserted CLC-K/barttin channels. |
| Function | Regulatory subunit of anion-selective CLCNKA:BSND and CLCNKB:BSND heteromeric channels involved in basolateral chloride conductance along the nephron to achieve urine concentration and maintain systemic acid-base homeostasis, and in the stria vascularis of the inner ear to establish the endocochlear potential necessary for normal hearing. Most likely acts as a chaperone that allosterically regulates proper sorting of CLCNKA:BSND and CLCNKB:BSND channels at the basolateral plasma membrane domain and functional switch to ion conducting state. Mediates constitutive opening of channel common gates. |
| Protein Name | Barttin |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Basolateral Cell MembraneMulti-Pass Membrane ProteinMostly Sorted At The Basolateral MembraneA Significant Amount Also Observed IntracellularlyStaining In Membranes Of The Renal Tubule And Of Potassium-Secreting Epithelia Of The Inner Ear Is Basolateral |
| Alternative Antibody Names | Anti-Barttin antibodyAnti-BSND antibodyAnti-BART antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
