STJ70304 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-BS69/ZMYND11 antibody (N-Term) (STJ70304)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70304-100
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General Information

Short DescriptionGoat polyclonal antibody anti-BS69/ZMYND11 (N-Term) is suitable for use in ELISA and Immunofluorescence research applications.
ApplicationsPep-ELISA/IF
HostGoat
ReactivityHuman/Mouse/Rat/Dog/Pig/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
IF: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ยตg/ml.
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolZMYND11
Gene ID10771
Uniprot IDZMY11_HUMAN
Accession NumberNP_006615.2; NP_997644.2; NP_001189393.1; NP_001189394.1; NP_001189395.1; NP_001189396.1; NP_001189397.1
Immunogen RegionN-Term
Immunogen SequenceSRVHGMHPKETT
SpecificityThis antibody is expected to recognise all reported human isoforms (NP_006615.2; NP_997644.2; NP_001189393.1; NP_001189394.1; NP_001189395.1; NP_001189396.1; NP_001189397.1).

Additional Info

Post Translational Modifications Sumoylated following its interaction with PIAS1 and UBE2I. Ubiquitinated, leading to proteasomal degradation.
Function Chromatin reader that specifically recognizes and binds histone H3.3 trimethylated at 'Lys-36' (H3.3K36me3) and regulates RNA polymerase II elongation. Does not bind other histone H3 subtypes (H3.1 or H3.2). Colocalizes with highly expressed genes and functions as a transcription corepressor by modulating RNA polymerase II at the elongation stage. Binds non-specifically to dsDNA. Acts as a tumor-suppressor by repressing a transcriptional program essential for tumor cell growth. (Microbial infection) Inhibits Epstein-Barr virus EBNA2-mediated transcriptional activation and host cell proliferation, through direct interaction.
Protein Name Zinc Finger Mynd Domain-Containing Protein 11
Adenovirus 5 E1a-Binding Protein
Bone Morphogenetic Protein Receptor-Associated Molecule 1
Protein Bs69
Cellular Localisation Nucleus
Chromosome
Associates With Chromatin And Mitotic Chromosomes
Alternative Antibody Names Anti-Zinc Finger Mynd Domain-Containing Protein 11 antibody
Anti-Adenovirus 5 E1a-Binding Protein antibody
Anti-Bone Morphogenetic Protein Receptor-Associated Molecule 1 antibody
Anti-Protein Bs69 antibody
Anti-ZMYND11 antibody
Anti-BRAM1 antibody
Anti-BS69 antibody

Information sourced from Uniprot.org

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