Anti-BRSK1 antibody (710-750 aa) (STJ13100765)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide to from aa region 710-750 of mouse BRSK1 conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-BRSK1 (710-750 aa) for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | BRSK1 |
| Gene ID | 84446 |
| Uniprot ID | BRSK1_HUMAN |
| Immunogen | A synthetic peptide to from aa region 710-750 of mouse BRSK1 conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | 710-750 aa |
| Specificity | Specific for BRSK1. |
Additional Info
| Post Translational Modifications | Phosphorylated at Thr-189 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not phosphorylated at Thr-189 by CaMKK2. In contrast, it is phosphorylated and activated by CaMKK1. May be inactivated via dephosphorylation of Thr-189 by PP2C. |
| Function | Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C. |
| Protein Name | Serine/Threonine-Protein Kinase Brsk1Brain-Selective Kinase 1Brain-Specific Serine/Threonine-Protein Kinase 1Br Serine/Threonine-Protein Kinase 1Serine/Threonine-Protein Kinase Sad-BSynapses Of Amphids Defective Homolog 1Sad1 HomologHsad1 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusCytoskeletonMicrotubule Organizing CenterCentrosomeSynapsePresynaptic Active ZoneCytoplasmic VesicleSecretory VesicleSynaptic VesicleNuclear In The Absence Of Dna DamageTranslocated To The Nucleus In Response To Uv- Or Mms-Induced Dna Damage |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Brsk1 antibodyAnti-Brain-Selective Kinase 1 antibodyAnti-Brain-Specific Serine/Threonine-Protein Kinase 1 antibodyAnti-Br Serine/Threonine-Protein Kinase 1 antibodyAnti-Serine/Threonine-Protein Kinase Sad-B antibodyAnti-Synapses Of Amphids Defective Homolog 1 antibodyAnti-Sad1 Homolog antibodyAnti-Hsad1 antibodyAnti-BRSK1 antibodyAnti-KIAA1811 antibodyAnti-SAD1 antibodyAnti-SADB antibody |
Information sourced from Uniprot.org