• Immunofluorescence analysis of U-2 OS cells using BRPF1 Rabbit polyclonal antibody (STJ11102828) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using BRPF1 Rabbit polyclonal antibody (STJ11102828) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using BRPF1 Rabbit polyclonal antibody (STJ11102828) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse testis, using BRPF1 Rabbit polyclonal antibody (STJ11102828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BRPF1 antibody (1-200) (STJ11102828)

SKU:
STJ11102828

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRPF1 (1-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRPF1
Gene ID: 7862
Uniprot ID: BRPF1_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BRPF1 (NP_001003694).
Immunogen Sequence: MGVDFDVKTFCHNLRATKPP YECPVETCRKVYKSYSGIEY HLYHYDHDNPPPPQQTPLRK HKKKGRQSRPANKQSPSPSE VSQSPGREVMSYAQAQRMVE VDLHGRVHRISIFDNLDVVS EDEEAPEEAPENGSNKENTE TPAATPKSGKHKNKEKRKDS NHHHHHNVSASTTPKLPEVV YRELEQDTPDAPPRPTSYYR
Tissue Specificity High levels in testis.
Post Translational Modifications Acetylated by KAT6A.
Function Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity. Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac). Some HAT complexes preferentially mediate histone H3 'Lys-23' (H3K23ac) acetylation. Positively regulates the transcription of RUNX1 and RUNX2.
Protein Name Peregrin
Bromodomain And Phd Finger-Containing Protein 1
Protein Br140
Database Links Reactome: R-HSA-3214847
Reactome: R-HSA-6804758
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Localization To The Nucleus Depends On Kat6a
Ing5 And Meaf6
Localizes To Transcription Start Sites
Alternative Antibody Names Anti-Peregrin antibody
Anti-Bromodomain And Phd Finger-Containing Protein 1 antibody
Anti-Protein Br140 antibody
Anti-BRPF1 antibody
Anti-BR140 antibody

Information sourced from Uniprot.org

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