Anti-BRM antibody (Internal) (STJ73748)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-BRM (Internal) is suitable for use in ELISA and Immunofluorescence research applications. |
| Applications | Pep-ELISA/IF |
| Host | Goat |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:1000.IF: Strong expression of the protein seen in the Nucleus and some staining in the Cytoplasm of HeLa and U251 cells. Recommended concentration: 10Β΅g/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20Β°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SMARCA2 |
| Gene ID | 6595 |
| Uniprot ID | SMCA2_HUMAN |
| Accession Number | NP_003061.3; NP_620614.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | PSNSQLEIEGNSS |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_003061.3; NP_620614.2) |
Additional Info
| Function | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. |
| Protein Name | Probable Global Transcription Activator Snf2l2Atp-Dependent Helicase Smarca2Brg1-Associated Factor 190bBaf190bProtein Brahma HomologHbrmSnf2-AlphaSwi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2 |
| Database Links | Reactome: R-HSA-3214858Reactome: R-HSA-8939243Reactome: R-HSA-9824585Reactome: R-HSA-9845323 |
| Cellular Localisation | NucleusLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Probable Global Transcription Activator Snf2l2 antibodyAnti-Atp-Dependent Helicase Smarca2 antibodyAnti-Brg1-Associated Factor 190b antibodyAnti-Baf190b antibodyAnti-Protein Brahma Homolog antibodyAnti-Hbrm antibodyAnti-Snf2-Alpha antibodyAnti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2 antibodyAnti-SMARCA2 antibodyAnti-BAF190B antibodyAnti-BRM antibodyAnti-SNF2A antibodyAnti-SNF2L2 antibody |
Information sourced from Uniprot.org