Anti-BRF2 antibody (180-419) (STJ116330)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BRF2 (180-419) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BRF2 |
| Gene ID | 55290 |
| Uniprot ID | BRF2_HUMAN |
| Immunogen Region | 180-419 |
| Immunogen Sequence | FKLFQASPSVPAKYVEDKEK MLSRTMQLVELANETWLVTG RHPLPVITAATFLAWQSLQP ADRLSCSLARFCKLANVDLP YPASSRLQELLAVLLRMAEQ LAWLRVLRLDKRSVVKHIGD LLQHRQSLVRSAFRDGTAEV ETREKEPPGWGQGQGEGEVG NNSLGLPQGKRPASPALLLP PCMLKSPKRICPVPPVSTVT GDENISDSEIEQYLRTPQEV RDFQRAQAARQAATSVPNP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 180-419 of human BRF2 (NP_060780.2). |
Additional Info
| Post Translational Modifications | In response to oxidative stress, Cys-361 is reversibly oxidized to cysteine sulfenic acid. Oxidation of Cys-361 impairs formation of a ternary complex with TBP and DNA and down-regulates expression of target genes in response to oxidative stress. |
| Function | General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression.functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress. |
| Protein Name | Transcription Factor Iiib 50 Kda SubunitTfiiib50Htfiiib50B-Related Factor 2Brf-2Hbrfu |
| Database Links | Reactome: R-HSA-749476Reactome: R-HSA-76071 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor Iiib 50 Kda Subunit antibodyAnti-Tfiiib50 antibodyAnti-Htfiiib50 antibodyAnti-B-Related Factor 2 antibodyAnti-Brf-2 antibodyAnti-Hbrfu antibodyAnti-BRF2 antibodyAnti-BRFU antibodyAnti-PRO1470 antibody |
Information sourced from Uniprot.org