• Anti-BRE antibody (51-229) (STJ117316)
  • Immunofluorescence analysis of paraffin-embedded human placenta using BRE Rabbit polyclonal antibody (STJ117316) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using BRE Rabbit polyclonal antibody (STJ117316) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using BRE Rabbit polyclonal antibody (STJ117316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BRE antibody (51-229) (STJ117316)

SKU:
STJ117316

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRE (51-229) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BABAM2
Gene ID: 9577
Uniprot ID: BABA2_HUMAN
Immunogen Region: 51-229
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 51-229 of human BRE (NP_954661.1).
Immunogen Sequence: PNCDRFKLHIPYAGETLKWD IIFNAQYPELPPDFIFGEDA EFLPDPSALQNLASWNPSNP ECLLLVVKELVQQYHQFQCS RLRESSRLMFEYQTLLEEPQ YGENMEIYAGKKNNWTGEFS ARFLLKLPVDFSNIPTYLLK DVNEDPGEDVALLSVSFEDT EATQVYPKLYLSPRIEHAL
Tissue Specificity Expressed in all cell lines examined. Highly expressed in placenta.
Function Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Within the BRISC complex, acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. The BRISC complex plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1.deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A.however these effects may be indirect.
Protein Name Brisc And Brca1-A Complex Member 2
Brca1-A Complex Subunit Bre
Brca1/Brca2-Containing Complex Subunit 45
Brain And Reproductive Organ-Expressed Protein
Database Links Reactome: R-HSA-5689901
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-69473
Cellular Localisation Cytoplasm
Nucleus
Localizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs)
Alternative Antibody Names Anti-Brisc And Brca1-A Complex Member 2 antibody
Anti-Brca1-A Complex Subunit Bre antibody
Anti-Brca1/Brca2-Containing Complex Subunit 45 antibody
Anti-Brain And Reproductive Organ-Expressed Protein antibody
Anti-BABAM2 antibody
Anti-BRCC45 antibody
Anti-BRE antibody

Information sourced from Uniprot.org

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