![Western blot analysis of lysates from wild type (WT) and BRD7 knockout (KO) 293T cells, using [KO Validated] BRD7 Rabbit polyclonal antibody (STJ22836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/99228/383979/STJ22836_1__90057.1713152605.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BRD7 knockout (KO) 293T cells, using [KO Validated] BRD7 Rabbit polyclonal antibody (STJ22836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western blot analysis of various lysates using [KO Validated] BRD7 Rabbit polyclonal antibody (STJ22836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/99228/383980/STJ22836_2__17805.1713152606.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] BRD7 Rabbit polyclonal antibody (STJ22836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of lysates from wild type (WT) and BRD7 knockout (KO) 293T cells, using [KO Validated] BRD7 Rabbit polyclonal antibody (STJ22836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/99228/383979/STJ22836_1__90057.1713152605.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BRD7 knockout (KO) 293T cells, using [KO Validated] BRD7 Rabbit polyclonal antibody (STJ22836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
Anti-BRD7 antibody (1-95) (STJ22836)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BRD7 (1-95) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BRD7 |
| Gene ID | 29117 |
| Uniprot ID | BRD7_HUMAN |
| Immunogen Region | 1-95 |
| Immunogen Sequence | MGKKHKKHKSDKHLYEEYVE KPLKLVLKVGGNEVTELSTG SSGHDSSLFEDKNDHDKHKD RKRKKRKKGEKQIPGEEKGR KRRRVKEDKKKRDRD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human BRD7 (NP_037395.2). |
Additional Info
| Function | Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR. Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase. |
| Protein Name | Bromodomain-Containing Protein 775 Kda Bromodomain ProteinProtein Celtix-1 |
| Database Links | Reactome: R-HSA-6804758 |
| Cellular Localisation | NucleusChromosomeIsoform 2: Nucleus |
| Alternative Antibody Names | Anti-Bromodomain-Containing Protein 7 antibodyAnti-75 Kda Bromodomain Protein antibodyAnti-Protein Celtix-1 antibodyAnti-BRD7 antibodyAnti-BP75 antibodyAnti-CELTIX1 antibody |
Information sourced from Uniprot.org