• Western blot analysis of various lysates, using BRD7 Rabbit polyclonal antibody (STJ11105077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BRD7 antibody (1-95) (STJ11105077)

SKU:
STJ11105077

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bromodomain-Containing Protein 7 (1-95) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRD7
Gene ID: 29117
Uniprot ID: BRD7_HUMAN
Immunogen Region: 1-95
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human BRD7. (NP_037395.2).
Immunogen Sequence: MGKKHKKHKSDKHLYEEYVE KPLKLVLKVGGNEVTELSTG SSGHDSSLFEDKNDHDKHKD RKRKKRKKGEKQIPGEEKGR KRRRVKEDKKKRDRD
Function Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR. Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
Protein Name Bromodomain-Containing Protein 7
75 Kda Bromodomain Protein
Protein Celtix-1
Database Links Reactome: R-HSA-6804758
Cellular Localisation Nucleus
Chromosome
Isoform 2: Nucleus
Alternative Antibody Names Anti-Bromodomain-Containing Protein 7 antibody
Anti-75 Kda Bromodomain Protein antibody
Anti-Protein Celtix-1 antibody
Anti-BRD7 antibody
Anti-BP75 antibody
Anti-CELTIX1 antibody

Information sourced from Uniprot.org

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