Anti-BRD6/BRDT antibody (C-Term) (STJ70303)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-BRD6/BRDT (C-Term) is suitable for use in ELISA research applications. |
| Applications | Pep-ELISA |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 75kDa band in human testis lysate after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | BRDT |
| Gene ID | 676 |
| Uniprot ID | BRDT_HUMAN |
| Accession Number | NP_001717.2; NP_997072.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | QSDIMTMFENNFD |
| Specificity | Variants (NP_001717.2; NP_997072.1) encode the same protein. |
Additional Info
| Function | Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin. |
| Protein Name | Bromodomain Testis-Specific ProteinCancer/Testis Antigen 9Ct9Ring3-Like Protein |
| Cellular Localisation | NucleusDetected On Chromatin |
| Alternative Antibody Names | Anti-Bromodomain Testis-Specific Protein antibodyAnti-Cancer/Testis Antigen 9 antibodyAnti-Ct9 antibodyAnti-Ring3-Like Protein antibodyAnti-BRDT antibody |
Information sourced from Uniprot.org