• Immunohistochemistry analysis of BRD3 in paraffin-embedded rat testis using BRD3 Rabbit polyclonal antibody (STJ111143) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using BRD3 Rabbit polyclonal antibody (STJ111143) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-BRD3 antibody (150-305) (STJ111143)

SKU:
STJ111143

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRD3 (150-305) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRD3
Gene ID: 8019
Uniprot ID: BRD3_HUMAN
Immunogen Region: 150-305
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 150-305 of human BRD3 (NP_031397.1).
Immunogen Sequence: PAPKGKGRKPAAGAQSAGTQ QVAAVSSVSPATPFQSVPPT VSQTPVIAATPVPTITANVT SVPVPPAAAPPPPATPIVPV VPPTPPVVKKKGVKRKADTT TPTTSAITASRSESPPPLSD PKQAKVVARRESGGRPIKPP KKDLEDGEVPQHAGKK
Tissue Specificity Ubiquitous.
Function Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets.
Protein Name Bromodomain-Containing Protein 3
Ring3-Like Protein
Cellular Localisation Nucleus
Detected On Chromatin
Alternative Antibody Names Anti-Bromodomain-Containing Protein 3 antibody
Anti-Ring3-Like Protein antibody
Anti-BRD3 antibody
Anti-KIAA0043 antibody
Anti-RING3L antibody

Information sourced from Uniprot.org

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