| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-B-Raf (N-Term) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:1000IHCICCIP |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BRAF |
| Gene ID: | 673 |
| Uniprot ID: | BRAF_HUMAN |
| Immunogen Region: | N-Term |
| Specificity: | The antibody detects 105 and 95 kDa* bands corresponding to the molecular mass of B-Raf. The doublet observed in Jurkat cells may represent multipe isoforms of the B-Raf, since all bands are blocked specifically with B-Raf (N-terminus) peptide. |
| Immunogen: | A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminus of human B-Raf. This sequence has high homology with similar regions in rat and mouse B-Raf, and has low homology to other Raf family members. |
| Post Translational Modifications | Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149.which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
| Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. |
| Protein Name | Serine/Threonine-Protein Kinase B-RafProto-Oncogene B-RafP94V-Raf Murine Sarcoma Viral Oncogene Homolog B1 |
| Database Links | Reactome: R-HSA-1295596Reactome: R-HSA-170968Reactome: R-HSA-170984Reactome: R-HSA-187706Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-5675221Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9726840Reactome: R-HSA-9726842 |
| Cellular Localisation | NucleusCytoplasmCell MembraneColocalizes With Rgs14 And Raf1 In Both The Cytoplasm And Membranes |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase B-Raf antibodyAnti-Proto-Oncogene B-Raf antibodyAnti-P94 antibodyAnti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 antibodyAnti-BRAF antibodyAnti-BRAF1 antibodyAnti-RAFB1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
