• Western blot analysis of extracts of various cell lines, using BPGM antibody (STJ110190) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of HeLa cells using BPGM Rabbit polyclonal antibody (STJ110190) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using BPGM rabbit polyclonal antibody (STJ110190) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using BPGM antibody (STJ110190) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BPGM antibody (1-259) (STJ110190)

SKU:
STJ110190

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BPGM (1-259) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BPGM
Gene ID: 669
Uniprot ID: PMGE_HUMAN
Immunogen Region: 1-259
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human BPGM (NP_001715.1).
Immunogen Sequence: MSKYKLIMLRHGEGAWNKEN RFCSWVDQKLNSEGMEEARN CGKQLKALNFEFDLVFTSVL NRSIHTAWLILEELGQEWVP VESSWRLNERHYGALIGLNR EQMALNHGEEQVRLWRRSYN VTPPPIEESHPYYQEIYNDR RYKVCDVPLDQLPRSESLKD VLERLLPYWNERIAPEVLRG KTILISAHGNSSRALLKHLE GISDEDIINITLPTGVPILL ELDENLRAVGPHQFLGDQE
Tissue Specificity Expressed in red blood cells. Expressed in non-erythroid cells of the placenta.present in the syncytiotrophoblast layer of the placental villi at the feto-maternal interface (at protein level).
Post Translational Modifications Glycation of Lys-159 in diabetic patients inactivates the enzyme.
Function Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase activity.
Protein Name Bisphosphoglycerate Mutase
Bpgm
2 -3-Bisphosphoglycerate Mutase - Erythrocyte
2 -3-Bisphosphoglycerate Synthase
2 -3-Diphosphoglycerate Mutase
Dpgm
Bpg-Dependent Pgam
Database Links Reactome: R-HSA-70171
Alternative Antibody Names Anti-Bisphosphoglycerate Mutase antibody
Anti-Bpgm antibody
Anti-2 -3-Bisphosphoglycerate Mutase - Erythrocyte antibody
Anti-2 -3-Bisphosphoglycerate Synthase antibody
Anti-2 -3-Diphosphoglycerate Mutase antibody
Anti-Dpgm antibody
Anti-Bpg-Dependent Pgam antibody
Anti-BPGM antibody

Information sourced from Uniprot.org

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