• Immunohistochemistry analysis of BOLA3 in paraffin-embedded Rat ovary using BOLA3 Rabbit polyclonal antibody (STJ118430) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BOLA3 in paraffin-embedded Human oophoroma using BOLA3 Rabbit polyclonal antibody (STJ118430) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using BOLA3 Rabbit polyclonal antibody (STJ118430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-BOLA3 antibody (1-68) (STJ118430)

SKU:
STJ118430

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BOLA3 (1-68) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BOLA3
Gene ID: 388962
Uniprot ID: BOLA3_HUMAN
Immunogen Region: 1-68
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human BOLA3 (NP_997717.2).
Immunogen Sequence: MAAWSPAAAAPLLRGIRGLP LHHRMFATQTEGELRVTQIL KEKFPRATAIKVTDISGGCG AMYEIKIE
Tissue Specificity Widely expressed.
Function Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates (Fe-S) cluster insertion into a subset of mitochondrial proteins. Probably acts together with NFU1.
Protein Name Bola-Like Protein 3
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Bola-Like Protein 3 antibody
Anti-BOLA3 antibody

Information sourced from Uniprot.org

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