• Western blot analysis of extracts of various cell lines, using BNIP1 Antibody (STJ29404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-BNIP1 antibody (1-200) (STJ29404)

SKU:
STJ29404

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BNIP1 (1-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BNIP1
Gene ID: 662
Uniprot ID: SEC20_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BNIP1 (NP_001196.2).
Immunogen Sequence: MAAPQDVHVRICNQEIVKFD LEVKALIQDIRDCSGPLSAL TELNTKVKEKFQQLRHRIQD LEQLAKEQDKESEKQLLLQE VENHKKQMLSNQASWRKANL TCKIAIDNLEKAELLQGGDL LRQRKTTKESLAQTSSTITE SLMGISRMMAQQVQQSEEAM QSLVTSSRTILDANEEFKSM SGTIQLGRKLITKYNRRELT
Tissue Specificity Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine.
Post Translational Modifications Polyubiquitinated. 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1. Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion.
Function As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Also plays a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable).
Protein Name Vesicle Transport Protein Sec20
Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 1
Transformation-Related Gene 8 Protein
Trg-8
Database Links Reactome: R-HSA-6811434
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Mitochondrion Membrane
Localization To The Mitochondrion Is Regulated By Rnf186
Alternative Antibody Names Anti-Vesicle Transport Protein Sec20 antibody
Anti-Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 1 antibody
Anti-Transformation-Related Gene 8 Protein antibody
Anti-Trg-8 antibody
Anti-BNIP1 antibody
Anti-NIP1 antibody
Anti-SEC20L antibody
Anti-TRG8 antibody

Information sourced from Uniprot.org

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