• Immunofluorescence analysis of U-2 OS cells using BMP6 Rabbit monoclonal antibody (STJ11102098) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using BMP6 Rabbit monoclonal antibody (STJ11102098) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using BMP6 Rabbit monoclonal antibody (STJ11102098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BMP6 antibody (350-450) [S8MR] (STJ11102098)

SKU:
STJ11102098

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-BMP6 (350-450) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BMP6
Gene ID: 654
Uniprot ID: BMP6_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human BMP6 (NP_001709.1).
Immunogen Sequence: DGPYDKQPFMVAFFKVSEVH VRTTRSASSRRRQQSRNRST QSQDVARVSSASDYNSSELK TACRKHELYVSFQDLGWQDW IIAPKGYAANYCDGECSFPL N
Post Translational Modifications Glycosylated at Asn-454. Glycosylation is crucial for recognition by the activin receptor type I/ACVR1.
Function Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes including cartilage and bone formation. Also plays an important role in the regulation of HAMP/hepcidin expression and iron metabolism by acting as a ligand for hemojuvelin/HJV. Also acts to promote expression of HAMP, potentially via the interaction with its receptor BMPR1A/ALK3. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target. Can also signal through non-canonical pathway such as TAZ-Hippo signaling cascade to modulate VEGF signaling by regulating VEGFR2 expression.
Protein Name Bone Morphogenetic Protein 6
Bmp-6
Vg-1-Related Protein
Vg-1-R
Vgr-1
Cellular Localisation Secreted
Alternative Antibody Names Anti-Bone Morphogenetic Protein 6 antibody
Anti-Bmp-6 antibody
Anti-Vg-1-Related Protein antibody
Anti-Vg-1-R antibody
Anti-Vgr-1 antibody
Anti-BMP6 antibody
Anti-VGR antibody

Information sourced from Uniprot.org

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