• Immunofluorescence analysis of MCF-7 cells using BRCC2/BLID Rabbit polyclonal antibody (STJ29414). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BRCC2/BLID, using BRCC2/BLID Rabbit polyclonal antibody (STJ29414) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BLID antibody (1-108) (STJ29414)

SKU:
STJ29414

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BLID (1-108) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BLID
Gene ID: 414899
Uniprot ID: BLID_HUMAN
Immunogen Region: 1-108
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human BRCC2/BLID (NP_001001786.2).
Immunogen Sequence: MVTLLPIEGQEIHFFEILES ECVLYTGWIERASGSSIYPE AKARLPLEALLGSNKEPMLP KETVLSLKRYNLGSSAMKRN VPGHVLQRPSYLTRIQVTLL CNSSAEAL
Tissue Specificity Ubiquitously expressed.
Function Functions as a proapoptotic molecule through the caspase-dependent mitochondrial pathway of cell death.
Protein Name Bh3-Like Motif-Containing Cell Death Inducer
Breast Cancer Cell Protein 2
Cellular Localisation Cytoplasm
Mitochondrion
Lower Abundance In Mitochondrion Than In Cytoplasm
Alternative Antibody Names Anti-Bh3-Like Motif-Containing Cell Death Inducer antibody
Anti-Breast Cancer Cell Protein 2 antibody
Anti-BLID antibody
Anti-BRCC2 antibody

Information sourced from Uniprot.org

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