Anti-BIRC6 antibody (STJA0004817)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide of human BIRC6
General Information
| Short Description | Rabbit polyclonal anti-Baculoviral Iap Repeat-Containing Protein 6 for use in IHC and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity purification |
| Dilution Range | IHC 1:50-1:200 |
| Formulation | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | BIRC6 |
| Gene ID | 57448 |
| Uniprot ID | BIRC6_HUMAN |
| Immunogen | Synthetic peptide of human BIRC6 |
Additional Info
| Tissue Specificity | Expressed in brain cancer cells. |
| Post Translational Modifications | Ubiquitinated.mediated by RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis. Deubiquitinated by USP8/UBPY. Autoubiquitinated.mediated by E1 ubiquitin activating enzyme UBA6. Proteolytically cleaved. Acts as substrate for CASP3, CASP6, CASP7, CASP9 and HTRA2. |
| Function | Anti-apoptotic protein known as inhibitor of apoptosis (IAP) which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Unlike most IAPs, does not contain a RING domain and it is not a RING-type E3 ligase. Instead acts as a dual E2/E3 enzyme that combines ubiquitin conjugating (E2) and ubiquitin ligase (E3) activities in a single polypeptide. Ubiquitination is mediated by a non-canonical E1 ubiquitin activating enzyme UBA6. Ubiquitinates CASP3, CASP7 and CASP9 and inhibits their caspase activity.also ubiquitinates their procaspases but to a weaker extent. Ubiquitinates pro-apoptotic factors DIABLO/SMAC and HTRA2. DIABLO/SMAC antagonizes the caspase inhibition activity of BIRC6 by competing for the same binding sites as the caspases. Ubiquitinates the autophagy protein MAP1LC3B.this activity is also inhibited by DIABLO/SMAC. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification. |
| Protein Name | Dual E2 Ubiquitin-Conjugating Enzyme/E3 Ubiquitin-Protein Ligase Birc6Bir Repeat-Containing Ubiquitin-Conjugating EnzymeBruceBaculoviral Iap Repeat-Containing Protein 6Ubiquitin-Conjugating Bir Domain Enzyme ApollonApollon |
| Database Links | Reactome: R-HSA-9700645Reactome: R-HSA-9725370 |
| Cellular Localisation | Golgi ApparatusTrans-Golgi Network MembraneEndosomeCytoplasmCytoskeletonSpindle PoleMicrotubule Organizing CenterCentrosomeMidbodyMidbody RingExhibits Cell Cycle-Dependent LocalizationConcentrates In A Pericentriolar Compartment In InterphaseMoves Partially To Spindle Poles In MetaphaseAnd Finally Localizes To The Spindle Midzone And The Midbody In Telophase And During CytokinesisOn The MidbodyLocalizes To The Midbody RingAlso Called Flemming BodyIn Interphase CellsLocalizes To The Trans-Golgi Network Membrane And EndosomesDuring CytokinesisA Fraction Moves To The Midzone Where It Specifically Arrives At The Midbody RingAfter Abscission CompletionTravels With The Midbody Remnant Into One Daughter CellAnd Remains Bound To It Until A New Midbody Ring Is Formed During The Next Cell Division |
| Alternative Antibody Names | Anti-Dual E2 Ubiquitin-Conjugating Enzyme/E3 Ubiquitin-Protein Ligase Birc6 antibodyAnti-Bir Repeat-Containing Ubiquitin-Conjugating Enzyme antibodyAnti-Bruce antibodyAnti-Baculoviral Iap Repeat-Containing Protein 6 antibodyAnti-Ubiquitin-Conjugating Bir Domain Enzyme Apollon antibodyAnti-Apollon antibodyAnti-BIRC6 antibodyAnti-KIAA1289 antibody |
Information sourced from Uniprot.org