![Anti-Biotin antibody [Hyb-8] (STJ16100225)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/148943/302024/STJ16100225_1__63437.1646940310.jpg?c=1 "Anti-Biotin antibody [Hyb-8] (STJ16100225)")
![Anti-Biotin antibody [Hyb-8] (STJ16100225)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/148943/302024/STJ16100225_1__63437.1646940310.jpg?c=1 "Anti-Biotin antibody [Hyb-8] (STJ16100225)")
Anti-Biotin antibody [Hyb-8] (STJ16100225)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenA BALB/c mice were immunized with biotinylated sheep immunoglobulin while NHS-biotin was used as a biotinylation agent. Fusion partner: SP2/0.
General Information
| Short Description | Mouse monoclonal anti-Biotin for use in ELISA, FC, WB, IF and IHC in samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/FC/WB/IF/IHC |
| Host | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | Hyb-8 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | ELISA (solid phase; not known; tracer: 0, 001-100 µg/ml for 30 min at RT). Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunoblotting (0, 5-1, 0 µg/ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT). |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Gene Symbol | HLCS |
| Gene ID | 3141 |
| Uniprot ID | BPL1_HUMAN |
| Immunogen | A BALB/c mice were immunized with biotinylated sheep immunoglobulin while NHS-biotin was used as a biotinylation agent. Fusion partner: SP2/0. |
Additional Info
| Tissue Specificity | Widely expressed. Mostly expressed in muscle, placenta and to a lower extent in the brain, kidney, pancreas, liver and lung. |
| Function | Biotin--protein ligase catalyzing the biotinylation of the 4 biotin-dependent carboxylases acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, and methylcrotonyl-CoA carboxylase. |
| Protein Name | Biotin--Protein LigaseBiotin Apo-Protein Ligase Includes - Biotin--Methylmalonyl-Coa-Carboxytransferase Ligase - Biotin--Propionyl-Coa-Carboxylase Atp-Hydrolyzing LigaseHolocarboxylase SynthetaseHcs - Biotin--Methylcrotonoyl-Coa-Carboxylase Ligase - Biotin--Acetyl-Coa-Carboxylase Ligase |
| Database Links | Reactome: R-HSA-196780Reactome: R-HSA-3371599 |
| Cellular Localisation | CytoplasmMitochondrion |
| Alternative Antibody Names | Anti-Biotin--Protein Ligase antibodyAnti-Biotin Apo-Protein Ligase Includes - Biotin--Methylmalonyl-Coa-Carboxytransferase Ligase - Biotin--Propionyl-Coa-Carboxylase Atp-Hydrolyzing Ligase antibodyAnti-Holocarboxylase Synthetase antibodyAnti-Hcs - Biotin--Methylcrotonoyl-Coa-Carboxylase Ligase - Biotin--Acetyl-Coa-Carboxylase Ligase antibodyAnti-HLCS antibody |
Information sourced from Uniprot.org