| Host: |
Mouse |
| Applications: |
ELISA/FC/WB/IF/IHC |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Biotin is suitable for use in ELISA, Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
Hyb-8 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1k |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
ELISA (solid phase; not known; tracer: 0, 001-100 µg/ml for 30 min at RT). Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunoblotting (0, 5-1, 0 µg/ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT). |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
HLCS |
| Gene ID: |
3141 |
| Uniprot ID: |
BPL1_HUMAN |
| Immunogen: |
A BALB/c mice were immunized with biotinylated sheep immunoglobulin while NHS-biotin was used as a biotinylation agent. Fusion partner: SP2/0. |
| Tissue Specificity | Widely expressed. Mostly expressed in muscle, placenta and to a lower extent in the brain, kidney, pancreas, liver and lung. |
| Function | Biotin--protein ligase catalyzing the biotinylation of the 4 biotin-dependent carboxylases acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, and methylcrotonyl-CoA carboxylase. |
| Protein Name | Biotin--Protein LigaseBiotin Apo-Protein Ligase Includes - Biotin--Methylmalonyl-Coa-Carboxytransferase Ligase - Biotin--Propionyl-Coa-Carboxylase Atp-Hydrolyzing LigaseHolocarboxylase SynthetaseHcs - Biotin--Methylcrotonoyl-Coa-Carboxylase Ligase - Biotin--Acetyl-Coa-Carboxylase Ligase |
| Database Links | Reactome: R-HSA-196780Reactome: R-HSA-3371599 |
| Cellular Localisation | CytoplasmMitochondrion |
| Alternative Antibody Names | Anti-Biotin--Protein Ligase antibodyAnti-Biotin Apo-Protein Ligase Includes - Biotin--Methylmalonyl-Coa-Carboxytransferase Ligase - Biotin--Propionyl-Coa-Carboxylase Atp-Hydrolyzing Ligase antibodyAnti-Holocarboxylase Synthetase antibodyAnti-Hcs - Biotin--Methylcrotonoyl-Coa-Carboxylase Ligase - Biotin--Acetyl-Coa-Carboxylase Ligase antibodyAnti-HLCS antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
