Anti-BHLHE41 antibody (120-280) (STJ112573)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BHLHE41 (120-280) antibody for use in WB and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BHLHE41 |
| Gene ID | 79365 |
| Uniprot ID | BHE41_HUMAN |
| Immunogen Region | 120-280 |
| Immunogen Sequence | LKSPIQSDLDAFHSGFQTCA KEVLQYLSRFESWTPREPRC VQLINHLHAVATQFLPTPQL LTQQVPLSKGTGAPSAAGSA AAPCLERAGQKLEPLAYCVP VIQRTQPSAELAAENDTDTD SGYGGEAEARPDREKGKGAG ASRVTIKQEPPGEDSPAPKR M |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human BHLHE41 (NP_110389.1). |
Additional Info
| Tissue Specificity | Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney. |
| Function | Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11. |
| Protein Name | Class E Basic Helix-Loop-Helix Protein 41Bhlhe41Class B Basic Helix-Loop-Helix Protein 3Bhlhb3Differentially Expressed In Chondrocytes Protein 2Hdec2Enhancer-Of-Split And Hairy-Related Protein 1Sharp-1 |
| Database Links | Reactome: R-HSA-1368108Reactome: R-HSA-400253 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Class E Basic Helix-Loop-Helix Protein 41 antibodyAnti-Bhlhe41 antibodyAnti-Class B Basic Helix-Loop-Helix Protein 3 antibodyAnti-Bhlhb3 antibodyAnti-Differentially Expressed In Chondrocytes Protein 2 antibodyAnti-Hdec2 antibodyAnti-Enhancer-Of-Split And Hairy-Related Protein 1 antibodyAnti-Sharp-1 antibodyAnti-BHLHE41 antibodyAnti-BHLHB3 antibodyAnti-DEC2 antibodyAnti-SHARP1 antibody |
Information sourced from Uniprot.org