• STJ73538 (0. 1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-BHLHE22 antibody (Internal) (STJ73538)

SKU:
STJ73538-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-BHLHE22 (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-Recommended concentration:0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: BHLHE22
Gene ID: 27319
Uniprot ID: BHE22_HUMAN
Immunogen Region: Internal
Accession Number: NP_689627.1
Immunogen Sequence: HKSLSASTSKRLE
Function Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA. In the brain, may function as an area-specific transcription factor that regulates the postmitotic acquisition of area identities and elucidate the genetic hierarchy between progenitors and postmitotic neurons driving neocortical arealization. May be required for the survival of a specific population of inhibitory neurons in the superficial laminae of the spinal cord dorsal horn that may regulate pruritis. Seems to play a crucial role in the retinogenesis, in the specification of amacrine and bipolar subtypes. Forms with PRDM8 a transcriptional repressor complex controlling genes involved in neural development and neuronal differentiation.
Protein Name Class E Basic Helix-Loop-Helix Protein 22
Bhlhe22
Class B Basic Helix-Loop-Helix Protein 5
Bhlhb5
Trinucleotide Repeat-Containing Gene 20 Protein
Database Links Reactome: R-HSA-9762293
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Class E Basic Helix-Loop-Helix Protein 22 antibody
Anti-Bhlhe22 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 5 antibody
Anti-Bhlhb5 antibody
Anti-Trinucleotide Repeat-Containing Gene 20 Protein antibody
Anti-BHLHE22 antibody
Anti-BHLHB5 antibody
Anti-TNRC20 antibody

Information sourced from Uniprot.org

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