![Western blot analysis of lysates from wild type (WT) and รยฒ2-microglobulin knockdown (KD) HeLa cells using [KD Validated] beta 2 Microglobulin Rabbit mAb (STJ11107311) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186507/408182/STJ11107311_1__43596.1744894577.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and รยฒ2-microglobulin knockdown (KD) HeLa cells using [KD Validated] beta 2 Microglobulin Rabbit mAb (STJ11107311) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.")
![Western blot analysis of various lysates using [KD Validated] beta 2 Microglobulin Rabbit mAb (STJ11107311) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186507/408183/STJ11107311_2__67231.1744894578.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] beta 2 Microglobulin Rabbit mAb (STJ11107311) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.")
![Western blot analysis of lysates from wild type (WT) and รยฒ2-microglobulin knockdown (KD) HeLa cells using [KD Validated] beta 2 Microglobulin Rabbit mAb (STJ11107311) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/186507/408182/STJ11107311_1__43596.1744894577.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and รยฒ2-microglobulin knockdown (KD) HeLa cells using [KD Validated] beta 2 Microglobulin Rabbit mAb (STJ11107311) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.")
Anti-beta 2 Microglobulin antibody (21-119 aa) [SRM5400] (STJ11107311)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal beta 2 Microglobulin (21-119 aa) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5400 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | B2M |
| Gene ID | 567 |
| Uniprot ID | B2MG_HUMAN |
| Immunogen Region | 21-119 aa |
| Immunogen Sequence | IQRTPKIQVYSRHPAENGKS NFLNCYVSGFHPSDIEVDLL KNGERIEKVEHSDLSFSKDW SFYLLYYTEFTPTEKDEYAC RVNHVTLSQPKIVKWDRDM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1). |
Additional Info
| Post Translational Modifications | Glycation of Ile-21 is observed in long-term hemodialysis patients. |
| Function | Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation. |
| Protein Name | Beta-2-Microglobulin Cleaved Into - Beta-2-Microglobulin Form Pi 5.3 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-1236977Reactome: R-HSA-164940Reactome: R-HSA-198933Reactome: R-HSA-2172127Reactome: R-HSA-2424491Reactome: R-HSA-6798695Reactome: R-HSA-877300Reactome: R-HSA-9637628Reactome: R-HSA-9705671Reactome: R-HSA-977225Reactome: R-HSA-983170 |
| Cellular Localisation | SecretedCell SurfaceDetected In Serum And Urine(Microbial Infection) In The Presence Of MTuberculosis Esxa-Esxb Complex Decreased Amounts Of B2m Are Found On The Cell Surface |
| Alternative Antibody Names | Anti-Beta-2-Microglobulin Cleaved Into - Beta-2-Microglobulin Form Pi 5.3 antibodyAnti-B2M antibodyAnti-CDABP0092 antibodyAnti-HDCMA22P antibody |
Information sourced from Uniprot.org